AGGREGATED CELL EVALUATION METHOD AND AGGREGATED CELL EVALUATION DEVICE

摘要

An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn.

主权项

1. An aggregated cell evaluation method comprising:
a speckle image acquisition step of irradiating aggregated cells with laser light and capturing speckle images generated by forward scattered light generated in the aggregated cells by irradiation with the laser light, at respective times t1 to tN in time series; an SC calculation step of calculating a speckle contrast value Kn of the speckle image at each time tn out of the times t1 to tN acquired in the speckle image acquisition step, determining a maximum value Kmax among the speckle contrast values K1 to KN, and normalizing each speckle contrast value Kn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′; and an evaluation step of evaluating motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn obtained in the SC calculation step or based on a correlation time τn or a speed Vn obtained therefrom.