DETECTION OF VIRAL DISEASES USING A BIOCHIP THAT CONTAINS GOLD NANOPARTICLES

摘要

A sensor for detecting molecular interactions between a target and a binding domain by electrochemical impedance spectroscopy. The sensor includes an anodic aluminum oxide barrier layer having a gold-coated array of regularly spaced nano-hemispheres and gold nanoparticles coated with a binding domain attached thereto. Also provided are methods for producing the sensor and for using the sensor to detect the presence of a virus in a sample.

主权项

1. A sensor for detecting molecular interactions between a target and a binding domain by electrochemical impedance spectroscopy, the sensor comprising:
a substrate, an anodic aluminum oxide (AAO) barrier layer attached to the substrate, the AAO barrier layer including an array of regularly spaced nano-hemispheres spaced apart by 5-30 nm, each nano-hemisphere having a diameter of 30-300 nm, an Au coating of 10-50 nm affixed to the AAO barrier layer, a plurality of gold nanoparticles (GNP) having a diameter of 2-10 nm deposited on the Au coating, and a binding domain attached to each GNP.