METHODS AND COMPOSITIONS FOR SINGLE CELL GENOMICS

摘要

Presented are methods and compositions for obtaining sequence information from one or more individual cells. The methods are useful for obtaining sequence information for a single nucleotide sequence, and for multiplex generation of sequence information from one or more individual cells.

主权项

1. A method of obtaining sequence information from an individual cell comprising:
(a) amplifying a nucleic acid molecule in situ within a cell, thereby generating an amplified nucleic acid molecule; (b) annealing in situ a barcode probe comprising at least a first decoder sequence and a second decoder sequence to said amplified nucleic acid molecule; (c) hybridizing a first labeled decoder probe to said first decoder sequence; (d) detecting a signal indicative of said hybridizing; (e) hybridizing a second labeled decoder probe to said second decoder sequence; and (f) detecting a signal indicative of said hybridizing, thereby generating sequence information regarding said amplified nucleic acid molecule.