Platelet Concentrating System

摘要

A method for more effectively concentrating blood platelets for use in medical procedures includes providing preparation and concentrating tubes. Anticoagulated whole blood is added to the preparation tube, which is centrifuged to separate red blood cells from a platelet plasma suspension The platelet plasma suspension is aspirated and loaded into the concentrating tube, which is itself centrifuged to separate the platelet plasma suspension into platelet poor plasma and platelet rich plasma layers. The platelet poor plasma is aspirated through a port of the concentrating tube and the remaining concentrated PRP is aspirated through the same or a different port of the concentrating tube.

主权项

1. A method of producing a platelet rich plasma (PRP) having a concentrated level of platelets and a reduced level of red blood cells, said method comprising:
providing a preparation centrifuge tube that includes an elongate receptacle having an interior chamber for receiving a blood product therein, which receptacle has closed upper and lower ends and a side wall extending between said upper and lower ends; providing a liquid impermeable sealing diaphragm within said chamber such that said diaphragm is longitudinally slidable through said chamber and maintains sealing interengagement with an interior surface of said side wall of said receptacle, said diaphragm separating said chamber into said upper and lower chamber regions, said preparation centrifuge tube further including a common inlet and outlet port formed through said receptacle and communicably connected to one of said upper and lower chamber regions, said preparation centrifuge tube further including a vent formed through said receptacle and communicably connected to the other of said upper and lower chamber regions for neutralizing air pressure within said receptacle; providing a concentrating tube that includes closed upper and lower end portions and a sidewall extending between said upper and lower end portions, said concentrating tube further having first and second aspiration ports formed through said upper end portion and communicably connected to an interior chamber of said concentrating tube, at least said first aspiration outlet having an aspiration pipe joined communicably thereto and extending to a distal nozzle at a predetermined depth within said chamber; introducing a blood product into one of said upper and lower chamber regions of said preparation tube through said common inlet and outlet port to drive said ceiling diaphragm longitudinally through said chamber; centrifuging said preparation tube at a speed and for a duration that separates the blood product into discrete fluid layers including an upper platelet plasma suspension layer and a lower red blood cell layer; aspirating said platelet plasma suspension layer from said preparation tube and introducing said platelet plasma suspension into the chamber of said concentrating tube; centrifuging the concentration tube at a speed and for a duration that separates the platelet plasma suspension into an upper platelet poor plasma layer containing a minority of the platelets from the platelet plasma suspension and a platelet rich plasma layer that includes a majority of the platelets from the platelet plasma suspension; aspirating the platelet poor plasma layer from said concentrating tube through said first aspirating port; and aspirating platelet rich plasma layer from the concentrating tube through said second aspirating port.