发明名称 |
TREATMENT OF A SAMPLE VESSEL |
摘要 |
Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads, and methods for treating sample vessels prior to recovery of converted DNA from the vessel. |
申请公布号 |
US2015368636(A1) |
申请公布日期 |
2015.12.24 |
申请号 |
US201414764716 |
申请日期 |
2014.01.30 |
申请人 |
EXACT SCIENCES CORPORATION |
发明人 |
Allawi Hatim;Lidgard Graham P.;Aizenstein Brian;Hunt Oliver;Zutz Tobias |
分类号 |
C12N15/10;C12Q1/68;C07H21/04 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for recovering nucleic acid from a sample vessel, comprising:
i) binding nucleic acid in a sample vessel; ii) recovering at least a portion of said nucleic acid from said sample vessel; wherein said sample vessel is exposed to a solution comprising at least one of bovine serum albumin or casein prior to said recovering. |
地址 |
Madison WI US |