USE OF TAQ POLYMERASE MUTANT ENZYMES FOR NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS

摘要

The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.

主权项

1. An isolated polypeptide comprising SEQ ID NO: 2 or a variant thereof having at least 85% sequence identity to SEQ ID NO: 2, wherein,
position 708 of the variant of SEQ ID NO: 2 is asparagine and the variant of SEQ ID NO: 2 has polymerase activity resistant to a polymerase chain reaction (PCR) inhibitor selected from the group consisting of blood, soil, and dye, or a combination thereof.