| 摘要 |
<p>Disclosed is a method for enzymatically regenerating the redox confactors NAD+/NADH and NADP+/NADPH in a one-pot reaction. In the method, one of the two redox cofactors is obtained in the reduced form thereof while the other redox cofactor is obtained in the oxidized form thereof as a result of at least two additional enzymatically catalyzed redox reactions (product forming reactions) taking place in the same reaction batch. The disclosed method is characterized in that a) oxygen or a compound of general formula R1C(O)COOH is reduced during the regeneration reaction converting the reduced cofactor back into the original oxidized form thereof, and b) a compound of general formula R2CH(OH)R3 is oxidized during the regeneration reaction converting the oxidized cofactor back into the original reduced form thereof. The process can be used in the epimerisation reactions of steroids, or the isomerisation of C5 or C6 sugars, such as reducing glucose to sorbitol and subsequently oxidising to fructose, while the simultaneous regeneration reaction recycles the utilised NAD+/NADH and NADP+/NADPH.</p> |
