| 发明名称 |
METHOD FOR SEPARATING CELL FROM BIOLOGICAL TISSUE |
| 摘要 |
Provided is a method for efficiently and stably separating cells having a high biological activity from a biological tissue, by using a degrading-enzyme composition, which is prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived from Clostridium sp. According to this method, the type and amount of protein-degrading enzyme to be used for isolating cells can be determined from the composition of a major protein of the biological tissue. Thus, cells having a high biological activity can be efficiently separated while reducing the amount of protein-degrading enzyme to be used. |
| 申请公布号 |
US2015368615(A1) |
申请公布日期 |
2015.12.24 |
| 申请号 |
US201414765020 |
申请日期 |
2014.01.31 |
| 申请人 |
TOHOKU UNIVERSITY ;MEIJI SEIKA PHARMA CO., LTD. ;NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY |
发明人 |
Goto Masafumi;Murayama Kazutaka;Yamagata Youhei;Watanabe Kimiko |
| 分类号 |
C12N5/071 |
主分类号 |
C12N5/071 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for separating cells from a biological tissue, comprising using a degrading-enzyme composition prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived from Clostridium sp. |
| 地址 |
Sendai-shi, Miyagi JP |
