Methods of treating amniotic membranes using supercritical fluids and compositions and apparatuses prepared therefrom

摘要

A method of sterilizing compositions prepared from amniotic membrane tissues may include harvesting placental tissue, separation of amniotic membrane tissue, and treatment of the amniotic membrane tissue with a supercritical fluid such as carbon dioxide. Treatment with supercritical fluid may subject the amniotic membrane tissue to conditions sufficient to sterilize the tissue yet maintain at least some biological function of the sterilized composition. The tissue preparations and compositions having a sterility assurance level of at least 10−6 described herein may be used as tissue grafts, wound dressings, cell culture substrates, or other substrates for use in tissue engineering.

主权项

1. A sterilized human tissue preparation for tissue engineering comprising a decellularized portion of a biological tissue comprising amniotic membrane, wherein the amniotic membrane is impregnated with a bioactive agent and has a sterility assurance level of 10−6.