MULTIMERIC ANTIMICROBIAL PEPTIDE COMPLEX WHICH IS DISPLAYED ON CELL SURFACE

摘要

The present invention provides an antimicrobial peptide polymer comprising at least one monomer which is digested by pepsin, a multimeric antimicrobial peptide complex comprising the polymer and a cell surface anchoring motif linked to the polymer, an antimicrobial microorganism displaying the multimeric antimicrobial peptide complex, an antimicrobial composition comprising the same, a method of treating an infectious disease caused by bacteria, yeast or fungi by administering the antimicrobial composition, and a method for producing the antimicrobial microorganism. According to the invention, living microorganisms displaying an antimicrobial peptide on the cell surface thereof may be administered in vivo without having to lyse the microbial cell and isolate and purify the antimicrobial peptide, so that the antimicrobial peptide exhibits antimicrobial activity. Thus, the antimicrobial peptide may be produced at significantly reduced costs so that it may have widespread use.

主权项

1. A method for producing antimicrobial microorganism displaying a multimeric antimicrobial peptide complex on the cell surface thereof, comprising
(a) preparing a recombinant vector comprising a polynucleotide encoding multimeric antimicrobial peptide complex comprising an antimicrobial peptide polymer and a cell surface anchoring motif linked to the polymer, wherein the antimicrobial peptide polymer comprises at least one monomer represented by the following formula 1 or 2:
N-terminus-[antimicrobial peptide-amino acid linker digested by pepsin]-C-terminus,  Formula 1N-terminus-[amino acid linker digested by pepsin antimicrobial peptide]-C-terminus,  Formula 2 wherein the antimicrobial peptide does not comprise leucine, phenylalanine, and tyrosine; the end of the amino acid linker that forms a peptide linkage with the terminus of the antimicrobial peptide is an amino acid selected from the group consisting of leucine, phenylalanine and tyrosine; the peptide linkage formed between the end of the linker and the terminus of the antimicrobial peptide is broken by the action of the digestive enzyme pepsin; and the cell surface anchoring motif is displayed on the cell surface of antimicrobial microorganism when being expressed in the microorganism. (b) introducing the recombinant vector into a host cell to obtain a transformant; and (c) culturing the transformant to induce the expression of the multimeric antimicrobial peptide complex.