TELECENTRIC , WIDE-FIELD FLUORESCENCE SCANNING SYSTEMS AND METHODS

摘要

Wide-field fluorescence imaging systems and methods. A bi-telecentric optical imaging system comprising imaging optics arranged and positioned such that a first telecentric space is created or exists between a sample platform and an entry aperture stop wherein Principal or chief rays from a plurality of field points on the sample platform are parallel to each other when passing through a first filter; and such that a second telecentric space is created or exists between a light detector and an exit aperture stop wherein the Principal or chief rays from the plurality of field points are parallel to each other when passing through a second filter. In this manner, light collected from different points in the field of view pass through the first filter at the same angles and also through the second filter at the same angles to thereby reduce or eliminate angular spectral shifting effects.

主权项

1. A fluorescence imaging system, comprising:
a source subsystem having:
a sample platform holding a fluorescent material;a light source that illuminates the fluorescent material with excitation light in an absorption band of the fluorescent material; anda first filter that passes wavelengths of light other than the excitation light, the first filter being positioned in a first light path between the sample platform and an entry aperture stop of a bi-telecentric optical imaging system; a detector subsystem for detecting light from the fluorescent material, comprising;
a light detector having an array of sensing locations; anda second filter that passes wavelengths of light in an emission band of the fluorescent material, the second filter being positioned in a second light path between the light detector and an exit aperture stop of the bi-telecentric optical imaging system; andthe bi-telecentric optical imaging system comprising imaging optics arranged and positioned such that a first telecentric space exists in the first light path between the sample platform and the entry aperture stop wherein Principal rays from a plurality of field points on the sample platform are parallel to each other when passing through the first filter; and such that a second telecentric space exists in the second light path between the light detector and the exit aperture stop wherein the Principal rays from the plurality of field points are parallel to each other when passing through the second filter.