Method for operating a light microscope and optical assembly

摘要

The invention relates to a method for operating a light microscope, which comprises at least the following components: a modulator diaphragm for restricting a light cross-section, a sample plane, which is located in a beam path behind the modulator diaphragm and in which a sample can be positioned, a phase ring, which is arranged in the beam path behind the sample plane and along the cross-section of which incident light can be differently influenced, and a pupil camera for capturing a pupil image. The method involves the following steps: with the aid of a beam splitter, which is located in the beam path in front of the phase ring, light that emanates from the sample is guided in part to the pupil camera and in part to the phase ring; a pupil image, which is not influenced by the phase ring, is recorded and a sample image is simultaneously emitted; with the aid of electronic image processing means in the recorded pupil image, the position and size of an image of the modulator diaphragm, which is influenced by a sample but not influenced by the phase ring, is measured; a relative displacement between the phase ring and the modulator diaphragm is performed on the basis of the measured position and of the measured size of the image of the modulator diaphragm with the aid of electronic control means. The invention further relates to an corresponding optical arrangement.

主权项

1. A method for operating a light microscope,
wherein the light microscope has at least the following components:
a modulator diaphragm for restricting a light cross-section,a specimen plane which is located in an optical path behind the modulator diaphragm and in which a specimen can be positioned,a phase ring which is arranged in the optical path behind the specimen plane and over the cross-section of which incident light is differently influenced, anda pupil camera for recording a pupil image, wherein with a beam splitter which is located in the optical path in front of the phase ring, light coming from the specimen is guided in part to the pupil camera and in part to the phase ring, wherein a pupil image which is uninfluenced by the phase ring is recorded and simultaneously a specimen image is output, wherein with electronic image processing means, the position and size of an image of the modulator diaphragm, which is influenced by a specimen and uninfluenced by the phase ring, are detected in the recorded pupil image, wherein a relative displacement between the phase ring and the modulator diaphragm is performed with electronic control means in dependence upon the detected position and the detected size of the image of the modulator diaphragm.