METHOD FOR PREPARING RECOMBINANT GLYCOPROTEIN HAVING HIGH SIALIC ACID CONTENT, THROUGH GLYCOSPHINGOLIPID SYNTHESIS CYCLE CONTROL

摘要

The present invention relates to a method for preparing a recombinant cell line producing recombinant glycoproteins having high sialic acid content by inhibiting glycosphingolipid (GSL) biosynthesis pathway in a cell line producing recombinant glycoproteins. Particularly, the present invention produces erythropoietin (EPO), containing a high content of sialic acid in a cell, from a cell line, which induces CGT inhibition by using siRNA and miRNA, specifically binding to ceramide glucosyltransferase (CGT), thereby increasing the in vivo half-life of a recombinant therapeutic protein, and thus can be useful in treatment using the same.