METHOD FOR DETECTING ENZYME ACTIVITY HYDROLYZING BETA-LACTAM RING ANTIMICROBIAL AGENTS

摘要

The present invention relates to a method for detecting an enzyme activity capable of hydrolyzing beta-lactam ring anti-microbial agents in a biological cell, comprising contacting said biological cell with at least one substrate of said enzyme activity comprising a beta-lactam ring, in an electrochemical cell, and detecting an impedance variation in said electrochemical cell with monitoring means. The present invention is in particular useful for detecting carbapenemase-producing Enterobacteriaceae (CPE).