发明名称 |
MULTIPLE DISPLACEMENT AMPLIFICATION |
摘要 |
The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample. |
申请公布号 |
EP2957641(A1) |
申请公布日期 |
2015.12.23 |
申请号 |
EP20150167348 |
申请日期 |
2010.10.15 |
申请人 |
IBIS BIOSCIENCES, INC. |
发明人 |
ESHOO, MARK W.;PICURI, JOHN;PHILLIPSON, CURTIS |
分类号 |
C12P19/34 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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