发明名称 |
FLUORESCENT AMP-KINASE BIOSENSORS |
摘要 |
The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP. |
申请公布号 |
CA2950051(A1) |
申请公布日期 |
2015.12.23 |
申请号 |
CA20152950051 |
申请日期 |
2015.06.18 |
申请人 |
UNIVERSITE GRENOBLE ALPES;EUROPEAN MOLECULAR BIOLOGY LABORATORY |
发明人 |
BERGER, IMRE;PELOSSE, MARTIN;SCHLATTNER, UWE |
分类号 |
C12N9/12;C12N5/10;C12N15/54;C12Q1/48;C12Q1/68;G01N33/52 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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