Process for production and quantitation of high yield of biobutanol

摘要

The present invention provides a process for production of high yields of butanol by Clostridium beijerinckii ATCC 10132. The process can be completed in a shorter span of time, using batch process through manipulation of various process parameters. The process can also be used for biomass based production of butanol. This paves the way for the strategic shift of major chemical industries from the hazardous routes of biofuel production to the biological, environmentally benign one.

主权项

1. A continuous process for production of butanol and acetone comprising: establishing and maintaining a culture in a fermentor, said culture comprising Clostridium beijerinckii ATCC 10132 and a nutrient medium comprising glucose, malt extract, and beef extract, wherein the concentration of the glucose in the nutrient medium is about 2%, the concentration of the malt extract in the nutrient medium is about 5%, and the concentration of the beef extract is about 5%; maintaining the concentrations of glucose, malt extract, beef extract and Clostridium beijerinckii ATCC 10132 in the fermentor at levels sufficient to maintain a continuous process; wherein the process is carried out without removing acetone or butanol from the fermenter; wherein the process is carried out at a temperature between 33° C. and 39°, at a pH in the range of 5.0 to 7.0 and with an inoculum density in the range of 1 to 2%; and wherein the process results in a yield of at least 20 g/L of butanol and the Clostridium beijerinckii ATCC 10132 tolerating a butanol concentration of about 2.5% in the nutrient medium.