Antibiotic-free plasmids

摘要

The present invention provides a method of maintaining a gram negative bacterium plasmid without the use of antibiotic selection pressure. Further, the invention relates to the drugless plasmids produced including drugless plasmids containing a heterologous gene. The invention also provides formulations and/or compositions comprising the drugless plasmids comprising a heterologous gene, formulations and/or compositions comprising a protein or an immunogen expressed using the drugless plasmids, and methods of administering such formulations and/or compositions to a host. The invention relates to gram negative bacteria containing the drugless plasmids.

主权项

1. A drugless plasmid comprising a polynucleotide encoding a cI repressor protein comprising the sequence of SEQ ID NO:2, wherein the expression of the cI repressor protein regulates the expression of a heterologous toxic gene product in a bacterial host.