Stabilized transglutaminase and process for production thereof

摘要

Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of: culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.

主权项

1. A process for producing a stabilized transglutaminase, the process comprising the steps of:
providing a microorganism transformed with a first polynucleotide encoding a transglutaminase pro-sequence peptide consisting of the amino acid sequence of SEQ ID NO: 1, 2, or 3 and further transformed with a second polynucleotide encoding a mature transglutaminase comprising the amino acid sequence of SEQ ID NO: 6; culturing the microorganism under suitable culture conditions to produce a polypeptide complex of the transglutaminase pro-sequence peptide noncovalently bound to the mature transglutaminase; and separating and recovering the polypeptide complex.