Magnetic lysis method and device

摘要

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.

主权项

1. A method for lysing cells within a vessel, said method comprising:
placing said vessel on a surface, wherein said vessel comprises one or more cells suspended in a liquid medium, one or more magnetic stirrers, and a plurality of beads; and rotating a cylinder shaped magnet along an axis, wherein said axis passes the center of said cylinder shaped magnet, is in the proximity of said vessel and is parallel to the surface said vessel resides on so that the vessel is within operational range of a rotating magnetic field produced by said cylinder shaped magnet, and wherein said rotating said cylinder shaped magnet causes said one or more magnetic stirrers in said vessel to collide with said beads with enough force to cause disintegration of said one or more cells, thereby lysing said one or more cells within said vessel.