Assays and methods of treatment relating to vitamin D insufficiency

摘要

Described herein are assays directed to determining the level of bioavailable or free vitamin D in a blood sample in a subject. The values determined for bioavailable or free vitamin D indicate whether the subject suffers from insufficient levels of vitamin D. Also described herein are methods of treatment for vitamin D insufficiency.

主权项

1. A method for determining a level of bioavailable vitamin D and a level of free vitamin D in a subject, the method comprising:
performing assays on a blood sample obtained from the subject to determine a level of VDBP (vitamin D binding protein) polypeptide, albumin polypeptide and total vitamin D; calculating a level of bioavailable vitamin D, wherein a level of bioavailable vitamin D is:
=(Kalb*[Alb]+1)*[Free Vitamin D] and calculating a level of free vitamin D, wherein a level of free vitamin D is:
={−{KDBP·[Total DBP]−KDBP·[Total Vitamin D]+Kalb·[Alb]+1}+√{(KDBP·[Total DBP]−KDBP·[Total Vitamin D]+Kalb·[Alb]+1)2+4·(KDBP·Kalb·[Alb]+KDBP)·([Total Vitamin D])}}÷(2·{KDBP·Kalb·[Alb]+KDBP}).