摘要 |
The invention relates to a primer combination for amplifying alpha chain CDR3 (complementary determining region 3) coding sequences of a T cell receptor and a use of the primer combination. The primer combination comprises a first primer set comprising at least one V region primer each of which comprises a sequence complementary with at least one V gene segment, and a second primer set comprising at least one J region primer each of which comprises a sequence complementary with at least one J gene segment. By virtue of the primer combination, the alpha chain CDR3 coding sequences of the T cell receptor can be effectively enriched, so that a convenient tool is provided for in-depth study of the alpha chain CDR3 of the T cell receptor. |