MAIZE EVENT DP-033121-3 AND METHODS FOR DETECTION THEREOF

摘要

The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-033121-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

主权项

1. A DNA construct comprising:
(a) a first expression cassette, comprising in operable linkage:
(i) a full length Citrus Yellow Mosaic virus (CYMV) promoter;(ii) a maize adh1 first intron;(iii) a synthetic chloroplast targeting peptide(iv) a Cry2A.127 encoding DNA molecule;(v) a ubiquitin3 (UBQ3) transcriptional terminator; and(vi) a 3′ untranslated region of an Arabidopsis ribosomal protein gene; (b) a second expression cassette, comprising in operable linkage:
(i) a truncated BSV promoter and second adh1 intron;(ii) a Cry1A.88 encoding DNA molecule; and(iii) a sorghum actin transcriptional terminator; (c) a third expression cassette, comprising in operable linkage:
(i) a maize polyubiquitin promoter;(ii) a 5′ untranslated region and intron1 of a maize polyubiquitin gene;(iii) a Vip3Aa20 encoding DNA molecule; and(iv) a pinII transcriptional terminator; and (d) a fourth expression cassette comprising in operable linkage:
(i) a maize polyubiquitin promoter;(ii) a mo-pat encoding DNA molecule; and(ii) a pinII transcriptional terminator.