COMPOSITIONS AND METHODS FOR THE AMPLIFICATION OF NUCLEIC ACIDS

摘要

The present disclosure relates to systems and methods for the amplification of nucleic acids, including, but not limited to, the amplification of nucleic acid libraries and whole genome amplification.

主权项

1. A method of creating a nucleic acid library from a nucleic acid sample, said method comprising:
a) providing a set of amplification primers to a nucleic acid sample, said set comprising
a plurality of random primers; anda plurality of locus specific primers configured to amplify a plurality of predetermined regions of said nucleic acid library; wherein said random primers are in greater abundance compared to said locus specific primers; and b) amplifying said nucleic acid sample using said set of amplification primers thereby creating a nucleic acid library.