Method For Delivering Desired Light Dose To Cells In A Light Attentuating Medium

摘要

A method provided for determining a range for the amount of light-energy attenuating material that may be present in a suspension containing target cells (such as MNCs), light-energy attenuating matter (such as RBCs and plasma), and a light-energy activatable compound (such as psoralen) so that a desired therapeutic effect (such as the percentage of MNCs in which apoptosis occurs) is obtained when the suspension is subjected to a known amount of light energy. In a related aspect, a method is provided for preparing a suspension containing target cells, light-energy attenuating matter, and a light-energy activatable compound so that a desired therapeutic effect is obtained when the suspension is subjected to a known amount of light energy

主权项

1. A photopheresis system comprising:
a disposable fluid circuit having a processing chamber for separating whole blood into one or more components including mononuclear cells and at least one treatment container for receipt of separated mononuclear cells having a predetermined thickness when a known volume of suspended mononuclear cells is received therein; a separation device adapted to receive the processing chamber for effecting separation of mononuclear cells from whole blood; an irradiation device adapted to receive the treatment container, and to subject the treatment container to a fixed emitted light dose; and a controller configured to control the hematocrit of the suspended mononuclear cells received in the treatment container.