SINGLE CELL CAPTURE WITH POLYMER CAPTURE FILMS

摘要

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a polymer capture film. In certain embodiments, the polymer capture films comprise a plurality of individual channels with top and bottom openings, where the channels are dimensioned such that a single cell is: i) is captured inside the channel, partially or substantially occluding the channel, when negative pressure is provided to the bottom opening; or ii) is captured by the top opening, but does not enter the channel, when negative pressure is provided to the bottom opening. In some embodiments, the channels of the polymer capture film align with the wells of a multi-well chip such that the cell, or the contents of the single cell, may be transferred to a corresponding well.

主权项

1. An article of manufacture comprising: a polymer capture film, wherein said polymer capture film comprises a top surface, a bottom surface, and a plurality of individual channels extending through said polymer capture film, wherein each of said individual channels:
a) has a top opening in said top surface of said polymer capture film, b) has a bottom opening in said bottom surface of said polymer capture film, and c) is dimensioned such that one cell, and only said one cell, from a cell suspension:
i) is captured inside said individual channel, partially or substantially occluding said individual channel, when negative pressure is provided to said bottom opening; orii) is captured by said top opening, but does not enter said individual channel, when negative pressure is provided to said bottom opening.