TRANSCRIPTION FACTOR-BASED GENERATION OF PACEMAKER CELLS AND METHODS OF USING SAME

摘要

Several embodiments disclosed herein relate generally to methods and compositions for the generation of biological pacemakers. In some embodiments, the methods comprise contacting non-pacemaker cells with one or more transcription factors (in vivo or in vitro) and inducing pacemaker functionality in the cells.

主权项

1. A method of generating a biological pacemaker using transcription factors to modify the electrical activity of the cardiac tissue of a subject comprising:
identifying a subject having cardiac tissue exhibiting abnormal electrical activity, wherein said subject has cardiac tissue comprising quiescent cells, wherein the quiescent cells comprise one or more of cardiomyocytes and stem cells, wherein the quiescent cells do not exhibit spontaneous, repetitive electrical activity; and administering one or more transcription factors to said quiescent cells to generate treated cells, wherein the treated cells exhibit spontaneous, repetitive electrical activity, thereby modifying electrical activity of the cardiac tissue said subject.