BIOSENSOR HAVING DECOUPLED CAPTURE CHAMBER AND DETECTION CHAMBER, USING PARTICLE AGGREGATION AND SIZE-SEPARATION

摘要

A biosensor using a decoupled microfluidic device, which has a capture chamber and a detection chamber separate from and in fluid communication with each other. The sensing method is based on particle aggregation via homogeneous reactions, by employing microparticles having antibodies on their surfaces which can form aggregates through antigen mediation. Either size-separation or magnetic based separation is used to separate aggregates from single microparticles; the aggregates are later dissociated and the resulting single microparticles are counted to measure the amount of the antigen. Another biosensor uses a decoupled microfluidic device with a capture chamber and a detection chamber, and a 3-D structure in the capture camber to increase immobilized antibody concentration. Immunoreaction efficiency is improved by increasing the number of antibody per reaction volume in the capture chamber.

主权项

1. A microfluidic device forming a biosensor, comprising:
a capture chamber; a detection chamber separate from and in fluid communication with the capture chamber, the detection chamber having a detection device for detecting single microparticles passing through the detection chamber; and a separation mechanism located in the capture chamber or between the capture chamber and the detection chamber, for separating aggregations of the microparticles and single microparticles.