SPECTROMETRY DEVICE, LIQUID CHROMATOGRAPH, AND WAVELENGTH CALIBRATION METHOD OF SPECTROMETER

摘要

The wavelength of a spectrometer is calibrated by using a commercial Ho glass filter. The spectrometer includes a light source including a D2 lamp and not including a mercury lamp, and a reference wavelength input unit for inputting, as a reference wavelength, a wavelength of a specific absorption peak separately measured for an Ho glass filter to be used. To calibrate the wavelength of the spectrometer by using the wavelength of a specific emission line peak of the D2 lamp and the reference wavelength input by the reference wavelength input unit, the wavelength calibration unit holds a conversion table showing a theoretical relationship between the number of control pulses for rotating a diffraction element and the corresponding wavelength of diffracted light, and calibrates the number of control pulses from the conversion table by the wavelength calibration unit.

主权项

1. A spectrometry device comprising:
a spectrometer including a diffraction element, a light source, including a D2 lamp and not including a mercury lamp, for generating incident light for the diffraction element, a light detection element arranged at a position for receiving light dispersed by the diffraction element, and a wavelength drive unit to be controlled by a control pulse to rotate the diffraction element; a filter attachment unit, in the spectrometer, for attaching an Ho glass filter in an attachable/detachable manner on a light path between the light source and the diffraction element; a reference wavelength input unit for inputting, as a reference wavelength, a wavelength of a specific absorption peak separately measured for the Ho glass filter; and a wavelength calibration unit including a conversion table holding unit for holding a conversion table showing a theoretical relationship between the number of control pulses for rotating the diffraction element and a corresponding wavelength of diffracted light, and a control pulse number calibration unit for calibrating the number of control pulses from the conversion table based on the number of control pulses n01 that is the number of control pulses obtained from the conversion table and that corresponds to a wavelength of a specific emission line peak of the D2 lamp, the number of control pulses n02 that is the number of control pulses obtained from the conversion table and that corresponds to the reference wavelength input by the reference wavelength input unit, the number of control pulses n1 before calibration at a time of detection of the emission line peak of the D2 lamp, and the number of control pulses n2 before calibration at a time of detection of the absorption peak of the Ho glass filter.