| 主权项 |
1: A method for liquid culturing (a) epithelial stem cells and/or (b) isolated epithelial tissue fragments comprising epithelial stem cells, the method comprising incubating the epithelial stem cells and/or the isolated tissue fragments in a liquid cell culture comprising a basal medium for animal or human cells to which is added (i) a Bone Morphogenetic Protein (BMP) inhibitor, (ii) a mitogenic growth factor, (iii) Wnt agonist, and (iv) at least about 4% w/v of extracellular matrix (ECM). |
