METHOD OF INHIBITING ANGIOGENESIS

摘要

A method of inhibiting angiogenesis by applying a kind of protein polysaccharide of a (1→6)-β-D-glucan obtained from a residue of extraction of crushed dried fruiting body of Agaricus blazei Murill (himematsutake) with 80% ethanol aqueous solution and hot water at 100 degrees C.

主权项

1. A method of inhibiting angiogenesis by applying a substance obtained by the steps of:
extracting a fruiting body of Agaricus blazei Murill (himematsutake) with a lower aliphatic alcohol or a lower aliphatic alcohol containing not more than 20% of water to obtain Residue S, extracting Residue S with hot water to obtain Residue I, extracting Residue I with 1% ammonium oxalate aqueous solution to obtain Residue II, extracting Residue II with 5% NaOH aqueous solution containing 0.05% NaBH4 and filtering to obtain Filtrate I, neutralizing Filtrate I with AcOH to pH of 5 to 6 to obtain Supernatant I, adding ethanol to Supernatant I to obtain Precipitate I, discarding compounds having molecular weight lower than 10,000 from Precipitate I and drying to obtain Fraction I, extracting Fraction I with 80% formic acid aqueous solution and filtering to obtain Filtrate II, fractionating Filtrate II by CHCl3-n-BuOH (5:1, v/v) to CHCl3-n-BuOH layer and Aqueous layer I, extracting Aqueous layer I with 5% lithium chloride-dimethylacetamide aqueous solution to obtain Precipitate II, and further fractionating Precipitate II by ethanol precipitation.