HLA-E BINDING

摘要

The invention relates to a method of testing a compound for biological activity, which method comprises providing cells expressing one of the CD94/NKG2 family of receptors, contacting the cells with recombinant HLA-E under binding conditions in the presence of the test compound, and determining whether the presence of the compound affects the binding of HLA-E to the cells. The HLA-E property of binding to CD94/NKG2 receptors on NK cells and a subset of CD8+ T cells is useful for targeting CD94/NKG2+ cells for a variety of purposes such as identification, isolation, killing or inactivation.

主权项

1. An in vitro method of protecting a cell or inhibiting HLA-E mediated inhibition of NK cell activity comprising:
(a) selecting an antibody that interferes with HLA-E-binding to an inhibitory CD94/NKG2 receptor from the group consisting of an anti-CD94 antibody, an anti-NKG2A antibody and an anti-HLA-E antibody; and (b) contacting an NK or T cell expressing an inhibitory CD94/NKG2 receptor with the antibody in vitro.