PROCESS FOR DETECTION OF DNA MODIFICATIONS AND PROTEIN BINDING BY A SINGLE MOLECULE MANIPULATION

摘要

The present invention relates to a method for determining whether a protein binds to a specific DNA sequence. This method is useful in particular for identifying modifications to the DNA sequence (e.g. methylations) via the binding of proteins that specifically recognize those modifications (e.g. antibodies), but also to identify the binding sequence on DNA of a variety of proteins.

主权项

1. A method for determining the binding of a protein to a nucleic acid molecule comprising a nucleic acid sequence, said method comprising:
a) denaturing a double-stranded nucleic acid molecule comprising a nucleic acid sequence by applying a physical force to the said molecule; b) providing said protein; c) renaturing said double stranded nucleic acid molecule in the presence of said protein; d) detecting a blockage of the re-naturation of the double-stranded nucleic acid; and e) determining the position of said blockage on said double-stranded nucleic acid molecule.