DYNAMIC BH3 PROFILING

摘要

The present invention provides methods of predicting cell sensitivity or resistance to a therapeutic agent.

主权项

1. A method of predicting sensitivity of a cell to a therapeutic agent comprising:
a) contacting a test cell population that has been exposed to a test therapeutic agent with a pro-apoptotic BH3 domain peptide; b) measuring the amount of BH3 domain peptide induced mitochondrial outer membrane permeabilization in the test cell population; and c) comparing the amount of BH3 domain peptide induced mitochondrial outer membrane membrane permeabilization in the test cell population to a control cell population that has not been contacted with the therapeutic agent, wherein an increase in mitochondrial outer membrane permeabilization in the test cell population compared to the control cell population indicates the cell is sensitive to the therapeutic agent.