PROTEIN BIOSENSORS, CROSS REACTIVE SENSOR ARRAYS AND METHODS OF USE THEREOF

摘要

The present invention is directed to fluorescent protein biosensors based on oligonucleotide cross reactive sensor arrays including a selective and a non-selective protein surface binding domain for protein detection. Interaction of different protein isoforms with the sensors of this invention yields a unique optical signature for each protein. The unique optical signature allows differentiating between closely related protein isoforms and diagnosing diseases and disorders associated with the proteins also in biofluids.

主权项

1. An oligodeoxyribose nucleotide (ODN) derivatives duplex, which consists of
a first ODN derivative comprising a first ODN sequence, at least one selective protein binder and a donor or acceptor; and a second ODN derivative comprising a second ODN sequence, which is complementary to said first ODN sequence, at least one non-selective protein surface binder and a donor or acceptor; wherein if one ODN derivative comprises a donor, the other one comprises an acceptor, and wherein at least one of the donor or acceptor is a fluorophore.