Method for rapid detection of mycotoxins (I). Method for rapid detection of mycotoxins (I) comprises: (1) providing a thin-layer waveguide (A) that has an optically transparent wave-conducting layer (a) deposited on a second optically transparent layer (b) where (b) has lower refractive index than (a), on which specific and/or affine binding partners (BP) and (bio)chemical recognition elements for (I) and/or the BP are immobilized in spatially separate locations; (2) applying a (I)-containing sample and binding partners (BP1) for BP to the immobilized BP on (A); (3) determining a signal in the evanescent field caused by interaction of the immobilized BP with (I) in the sample and/or BP1; and (4) determining the amount of (I) in the sample. Independent claims are included for the following: (1) device for the new method comprising the thin-layer waveguide as described; and (2) kit for detecting (I) comprising the waveguide and attached recognition elements.
