Devices and methods for detection of an analyte based upon light interference

摘要

Methods for analyzing an optical surface for an analyte of interest in a test sample and related instruments/devices are disclosed. The method entails the use of thin film optical immunoassay device whereby an analyte of interest is detected in a test sample through spectral changes in the light impinging the surface prior to and after the binding of the analyte to reactive substrate layer(s). The device includes a substrate which has a first color in response to light impinging thereon. The substrate also exhibits a second color which is different from the first color. The second color is exhibited in response to the same light when the analyte is present on the surface.