| 摘要 |
FIELD: medicine. ^ SUBSTANCE: invention refers to biotechnology. Hybridoma strain is prepared by immunisation of BALB/c mice with bovine HSP 70 within 78 days. On the third day, splenocyte hybridisation of immune mice (10 cells) with murine myeloma cells P3-X63 Ag/8-653 (107 cells) is carried out. As a fusion agent, polyethylene glycol of molecular weight 4000 (Merk, Germany) is applied. Hybridisation is followed with selection, screening, cloning and cryopreservation of hybridoma. ^ EFFECT: invention can be used for preparing monoclonal antibodies (MCA) to heat shock protein 70 (HSP 70). ^ 4 dwg, 1 tbl, 6 ex |
