发明名称 |
ENHANCEMENT OF SIRNA SILENCING ACTIVITY USING UNIVERSAL BASES OR MISMATCHES IN THE SENSE STRAND |
摘要 |
One aspect of the present invention relates to a double stranded nucleic acid useful as an siRNA, that has a sense strand and an antisense strand relative to a target nucleic acid, where the sense strand contains one or more modified nucleobases, or one or more mismatch base pairings with the antisense strand. Another aspect of the present invention relates to a single-stranded oligonucleotide comprising at least one nucleoside comprising a non-natural nucleobase. Another aspect of the invention relates to a method of gene silencing, comprising administering to a mammal in need thereof a therapeutically effective amount of a double-stranded oligonucleotides containing a sense strand and an antisense strand, where the sense strand contains one or more modified nucleobases, or one or more mismatch base pairings with the antisense strand. |
申请公布号 |
WO2010011895(A1) |
申请公布日期 |
2010.01.28 |
申请号 |
WO2009US51648 |
申请日期 |
2009.07.24 |
申请人 |
ALNYLAM PHARMACEUTICALS, INC.;MAIER, MARTIN;ADDEPALLI, HARIPRIYA;MANOHARAN, MUTHIAH |
发明人 |
MAIER, MARTIN;ADDEPALLI, HARIPRIYA;MANOHARAN, MUTHIAH |
分类号 |
A61K31/713;C07H21/00;C12N15/11 |
主分类号 |
A61K31/713 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|