发明名称 |
PREPARATIVE PURIFICATION PROCESS FOR HUMAN FURIN |
摘要 |
<p>Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50 -fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC„¢ resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC„¢ chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.</p> |
申请公布号 |
EP2147097(A2) |
申请公布日期 |
2010.01.27 |
申请号 |
EP20080758691 |
申请日期 |
2008.05.21 |
申请人 |
BAXTER INTERNATIONAL INC.;BAXTER HEALTHCARE S.A. |
发明人 |
MATTHIESSEN, PETER;ROMEDER-FINGER, STEFAN;TURECEK, PETER;SCHWARZ, HANS-PETER |
分类号 |
C12N9/64;A61K38/48;C07K1/18;C12N15/57 |
主分类号 |
C12N9/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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