发明名称 |
ONE-STEP TARGET DETECTION ASSAY |
摘要 |
The present invention provides nucleic acid amplification, detection, and genotyping techniques. In one embodiment, the present invention provides a method for amplifying and detecting a target nucleic acid sequence by providing a first primer pair comprising: a first primer comprising a target specific sequence, a tag sequence 5' of the target specific sequence, and a blocker between the target specific sequence and the tag sequence, and a second primer comprising a target specific sequence; providing a reporter attached to either the second primer or to a dNTP; providing a capture complex comprising an anti-tag sequence attached to a solid support; combining the first primer pair, the capture complex, the reporter, and a sample comprising a target nucleic acid sequence under conditions suitable for amplification of the target nucleic acid sequence and hybridization of the amplified target nucleic acid sequence to the capture complex; and detecting the amplified target nucleic acid sequence. |
申请公布号 |
WO2009074882(A2) |
申请公布日期 |
2009.06.18 |
申请号 |
WO2008IB03869 |
申请日期 |
2008.10.31 |
申请人 |
LUMINEX MOLECULAR DIAGNOSTICS, INC.;GALVAN-GOLDMAN, BARBARA;ZOLDOWSKI, AGNES;BORTOLIN, SUSAN;MERANTE, FRANCK |
发明人 |
GALVAN-GOLDMAN, BARBARA;ZOLDOWSKI, AGNES;BORTOLIN, SUSAN;MERANTE, FRANCK |
分类号 |
C12P19/34;C12Q1/68;C40B30/00;C40B30/04 |
主分类号 |
C12P19/34 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|