发明名称 |
Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
摘要 |
Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein. |
申请公布号 |
AU2004283299(B2) |
申请公布日期 |
2009.06.18 |
申请号 |
AU20040283299 |
申请日期 |
2004.10.22 |
申请人 |
KECK GRADUATE INSTITUTE;ALDER BIOPHARMACEUTICALS, INC. |
发明人 |
JAMES M. CREGG;ILYA I. TOLSTORUKOV;MARK LITTON;RANDALL SCHATZMAN;JOHN LATHAM |
分类号 |
C12N15/74;C07K16/00;C07K16/28;C12P21/04 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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