| 摘要 |
<p>Described is a method of the generation of cells using a mitochondrial targeted restriction endonuclease. This method comprises (a) transfecting cells with an expression vector containing a gene encoding a fusion protein comprising a mitochondrial targeting sequence (MTS) and a restriction endonuclease operatively linked to a suitable promoter; (b) culturing the transfected cells over a sufficient period of time; and (c) selecting cells, e.g., via FACS analysis.</p> |
