ELIMINATION OF THE BACTERIA THAT CAUSE PIERCE'S DISEASE

摘要

XyIeIIa fastidiosa is the cause of the Pierce's Disease in grapevines and other cultures of economic importance that are susceptible to the bacteria. Anti-Xylella fastidiosa substances were developed comprising hen scFv antibodies attached to a lytic peptide Cecropin Pl (CPI), a derivative of a nematode, expressed in M13 phages or in soluble form. The recombinant substance specifically eliminates 100% of the XyIeIIa fastidiosa in culture while using a concentration of 3.6 x IO(5) phages/bacterial in 28 hours of incubation. A scFv expressed in M13 phage eliminates 100% of the bacteria in a culture between 28 and 48 hours. A soluble scFv by itself or attached to the lytic peptide CP1 eliminates almost 100% of the XyIeIIa fastidiosa bacteria in culture. This strategy has a potential application to solve diseases in plants caused by bacteria.