POLYKETIDE SYNTHASE-NONRIBOSOMAL PEPTIDE SYNTHETASE GENE

摘要

Disclosed are: a method for detecting the genetic cyclopiazonic acid-producing ability of a strain belonging to the genus Aspergillus or the like rapidly and with high accuracy; a transformant (strain) incapable of producing cyclopiazonic acid or cycloacetoacetyl L-tryptophan (CAT) that is a precursor of cyclopiazonic acid; and others. Specifically disclosed is a polynucleotide encoding any one of the following polypeptides (1) to (3): (1) a polypeptide comprising the amino acid sequence depicted in SEQ ID NO:2; (2) a polypeptide which comprises an amino acid sequence having the deletion, substitution or addition of one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO:2, and which has a polyketide synthase-nonribosomal peptide synthetase activity; and (3) a polypeptide which has a 90% or more homology (identity) on total average with the amino acid sequence depicted in SEQ ID NO:2, and which has a polyketide synthase-nonribosomal peptide synthetase activity.