| 摘要 |
The present invention relates to the development of pseudobioaffinity adsorbent and process for purification of immunoglobulin G from immunoglobulin containing solutions such as but not limited to plasma, serum, cell culture supernatant, ascites fluids. The adsorbent consists of a solid support and ligand. The ligand may be attached to the matrix or be part of matrix. The ligand is selected from a group of hydrophobic amino acids such as alanine, valine, leucine, isoleucine, proline, phenylalanine, tryptophan and tyrosine and the support material being preferably a synthetic hydrophilic polymer of methacrylate or acrylate species, or any of its derivatives. The adsorbent is cheap and stable to harsh conditions such as 1.0 M NaOH used during regeneration of adsorbents. Moreover there is no problem of toxic leachables typically associated with biological ligands. The nature of the adsorbent also allows high flow rate operations at relatively low pressures. The process includes adjusting the pH and conductivity of the feed solution and the use of ionic salts and additives such as polyols or alcohols for eluting the bound IgG. |
