| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for detecting a target microorganism in good accuracy from a sample which contains a plurality of microorganisms. SOLUTION: Accurate detection of a target microorganism from a sample can be achieved by eliminating a possibility of cross-hybridization of a microorganism with other probes, which can accelerate the designing speed, detecting microorganisms one-by-one in the designed order of A, B, and C, and absorbing all the existing microorganisms. A method for detecting a nucleotide acid in a sample, includes: a first step 620, in which a single or a plurality of first probes capable of specifically capturing a particular nucleotide sequence out of a plurality of nucleic acid sequences potentially present in the sample are prepared and the first probes capture the nucleotide sequence in the sample; a second step 621, in which a plurality of or single second probes capable of specifically capturing a particular nucleotide sequence out of the plurality of nucleic acid sequences excluding the nucleic acid captured by the first probes are prepared and the second probes capture the nucleotide sequence in the sample having passed through the first step; and a step of detecting the presence or amount of at least one of nucleic acid sequences captured in the first step and the second step. COPYRIGHT: (C)2009,JPO&INPIT
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