| 摘要 |
The present invention provides a method of detecting a nucleic acid which is not restricted by the design of the base sequence of a nucleic acid probe. By repeating washing and detection in multiple stages, the present invention can improve the precision of sequence-specific hybridization stepwise and also can ease restrictions in designing the nucleic acid probes, in particular, restrictions on the Tm value (the temperature at which the nucleic acid double strand is dissociated into single strands) or the sequence length of the nucleic acid probes.
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