Platelet glycoprotein IB alpha variant fusion polypeptides and methods of use thereof

摘要

An isolated polypeptide comprising one or more substitutions in the amino acid sequence SEQ ID NO:2, wherein said one or more substitutions is Y276F; Y276FK237V; Y276FC65S; Y276FY278F Y279F; Y276FY278F Y279F K237V; or Y276FY278F Y279F K237V C65S, and wherein said polypeptide has at least one activity selected from the group consisting of: a) lower affinity binding to alpha thrombin relative to binding to alpha thrombin of a protein sequence with the amino acid sequence of SEQ ID NO: 2; b) lower aggregation relative to aggregation of a protein sequence with the amino acid sequence of SEQ ID NO: 2; and c) increased resistance to proteolysis relative to a protein sequence with the amino acid sequence of SEQ ID NO: 2. Also disclosed is a fusion protein encompassing said polypeptide and a method for the manufacture of a medicament.