A droplet actuator and method of providing a droplet comprising an target substance on the droplet actuator, and including discrete flow and continuous flow functionality. Discrete flow function is controlled by electrodes arranged for conducting droplet operations on a substrate surface. The continuous flow function includes a fluid path (101) arranged for flowing a fluid therethrough. The discrete flow and continuous flow functions may be separated by a barrier (103), including a second fluid path through the barrier. The continuous flow function may include a captux-e surface (501) comprising a component having an affinity for the target substance. Methods of the invention may include flowing a fluid comprising the target substance through the fluid path (101); capturing the target substance on the capture surface (501); and forming a droplet in the discrete flow region via the second fluid path and the electrodes comprising the captured target substance.
